Laboratory methods in clinical immunology for the 3rd year of study

Structura pe capitole: 1. METHODS FOR ASSESING IMMUNOLOGICAL MARKERS 2. METHODS FOR HIGHLIGHTING HYPERSENSITIVITY REACTIONS 3. MANUAL DNA EXTRACTION USING COLUMNS WITH SILICON DIOXIDE (QIAGEN) 4. AUTOMATIC DNA EXTRACTION WITH MAGNETIC BALLS 5. MEASUREMENT OF DNA CONCENTRATION AND PURITY (IMPLEN P300 NANOPHOTOMETER) 6. GENOTYPING OF HLA SSP- MEDIUM RESOLUTION AND INTERPRETATION OF HLA ALLELES 7. GENOTYPING OF HLA SSP BY HIGH RESOLUTION (INVITROGEN) SSP - METHOD AND INTERPRETATION OF HLA ALLELES 8. GENOTYPING OF HLA SBT ? SANGER METHOD (SECORE INVITROGEN) ?INTERPRETATION OF ALLELIC AMBIGUITY 9. HLA SSO-LUMINEX GENOTYPING WITH LABTYPE KITS (ONE LAMBDA) 10. GENOTYPING OF KIR GENES BY THE SSP METHOD 11. POLYMORPHISM OF CYTOKINE GENES BY THE SSP METHOD (PEL-FREEZ CYTOKINE GENOTYPING) 12. ELECTROPHORESIS IN AGAROSE GEL AND GEL PHOTOGRAPHY 13. EXTRACTION OF TOTAL RNA FROM TISSUE WITH PHENOL-ACID: CHLOROPHORM (INVITROGEN-MIRVANA MIRNA ISOLATION KIT) 14. REVERSE TRANSCRIPTION OF RNA 15. QUAN